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Empty Drops

Empty Drops is a filtering tool part of the library Droplet Utils. More information can be found at:

Empty Drops by Droplet Utils: Empty Drops .

Parameter Description Example
hto_raw path to filtered HTO matrix –hto_raw /”path/hto_raw/”
niters specifies the number of iterations to use for the Monte Carlo p-value calculations –niters 10000
lower specifies the lower bound on the total UMI count, at or below which all barcodes are assumed to correspond to empty droplets –lower 100.
testAmbient indicates whether results should be returned for barcodes with totals less than or equal to lower –testAmbient FALSE
alpha_empty specifies the scaling parameter for the Dirichlet-multinomial sampling scheme –alpha_empty NULL
ignore specifies the lower bound on the total UMI count, at or below which barcodes will be ignored –ignore NULL

The description of this parameters were provided by the library. They can also be found in the link above.