Empty Drops
Empty Drops is a filtering tool part of the library Droplet Utils. More information can be found at:
Empty Drops by Droplet Utils: Empty Drops .
Parameter | Description | Example |
---|---|---|
hto_raw | path to filtered HTO matrix | –hto_raw /”path/hto_raw/” |
niters | specifies the number of iterations to use for the Monte Carlo p-value calculations | –niters 10000 |
lower | specifies the lower bound on the total UMI count, at or below which all barcodes are assumed to correspond to empty droplets | –lower 100. |
testAmbient | indicates whether results should be returned for barcodes with totals less than or equal to lower | –testAmbient FALSE |
alpha_empty | specifies the scaling parameter for the Dirichlet-multinomial sampling scheme | –alpha_empty NULL |
ignore | specifies the lower bound on the total UMI count, at or below which barcodes will be ignored | –ignore NULL |
The description of this parameters were provided by the library. They can also be found in the link above.